Soil-borne plant pathogens characterize a critical risk that undermines industrial walnut (Juglans regia) manufacturing worldwide. Crown gall, attributable to Agrobacterium tumefaciens, and Phytophthora root and crown rots, attributable to varied Phytophthora spp., are among the many most devastating walnut soil-borne ailments.
A acknowledged technique to fight soil-borne ailments is adoption of resistant rootstocks. Right here, resistance to A. tumefaciens, P. cinnamomi, and P. pini is mapped within the genome of Juglans microcarpa, a North American wild relative of cultivated walnut. Half-sib J. microcarpa mom bushes DJUG 31.01 and DJUG 31.09 had been crossed with J. regia cv. Serr, producing 353 and 400 hybrids, respectively.
Clonally propagated hybrids had been genotyped by sequencing to assemble genetic maps for the 2 populations and challenged with the three pathogens. Resistance to every of the three pathogens was mapped as a serious QTL on the lengthy arm of J. microcarpa chromosome 4D and was related to the identical haplotype, designated as haplotype b, elevating the likelihood that the 2 mom bushes had been heterozygous for a single Mendelian gene conferring resistance to all three pathogens.
The deployment of this haplotype in rootstock breeding will facilitate breeding of a walnut rootstock immune to each crown gall and Phytophthora root and crown rots.
A number of Viral Infections Detected in Phytophthora condilina by Complete and Small RNA Sequencing
Marine oomycetes have lately been proven to be concurrently contaminated by (-)ssRNA viruses of the order Bunyavirales. On this work, even greater virus variability was present in a single isolate of Phytophthora condilina, a lately described member of Phytophthora phylogenetic Clade 6a, which was remoted from brackish estuarine waters in southern Portugal.
Utilizing complete and small RNA-seq the total RdRp of 13 totally different potential novel bunya-like viruses and two full toti-like viruses had been detected. All these viruses had been efficiently confirmed by reverse transcription polymerase chain response (RT-PCR) utilizing complete RNA as template, however complementarily one of many toti-like and 5 of the bunya-like viruses had been confirmed when dsRNA was purified for RT-PCR. In our examine, complete RNA-seq was by much more environment friendly for de novo assembling of the virus sequencing however small RNA-seq confirmed greater learn numbers for many viruses.
Two fundamental populations of small RNAs (21 nts and 25 nts-long) had been recognized, which had been in accordance with different Phytophthora species. To the very best of our information, that is the primary examine utilizing small RNA sequencing to determine viruses in Phytophthora spp.
Stepwise accumulation of mutations in CesA3 in Phytophthora sojae leads to rising resistance to CAA fungicides
Flumorph is a carboxylic acid amide (CAA) fungicide with excessive exercise towards oomycetes. Nonetheless, evolution to CAAs from low resistance to excessive resistance has by no means been reported. This examine investigated the premise of resistance evolution of flumorph in Phytophthora sojae.
Complete of 120 P. sojae isolates had been collected and their sensitivity to flumorph was evaluated. Though no spontaneous resistance was discovered among the many area isolates, adaptation on flumorph-amended media resulted within the collection of 5 steady mutant varieties exhibiting various levels of resistance to CAAs.
Kind I, which exhibited the bottom resistance stage, was obtained when the wild-type isolate was uncovered to a low focus of flumorph, however no resistant mutants had been obtained by direct publicity to greater concentrations. Nonetheless, the extra resistant varieties (Kind II, III, IV and V) had been obtained when Kind I had been uncovered to greater concentrations of flumorph.
Comparable outcomes had been obtained when your entire screening course of was repeated, which implied that evolution of resistance to flumorph in P. sojae could possibly be a two-step course of, the place excessive resistance phenotypes may develop regularly from low resistance ones.
Additional investigation into molecular mechanism strongly confirmed that evolution of isolates extremely immune to flumorph happens in a stepwise course of with Kind I as middleman, by means of accumulation of mutations of their goal protein of CAAs (CesA3). Collectively, our findings point out that software of low charges of flumorph in area may lead to collection of low resistance Kind I isolates, however that elevating dosage to keep up comparable ranges of management may elicit speedy evolution of extra resistant Kind II, III, IV and V isolates with stepwise accumulation of mutations in CesA3, which might render flumorph ineffective as a management technique.
Ginsenoside?Rh1) 20(R)Ginsenoside?Rh1 |
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| N2192-20 | ApexBio | 20 mg | EUR 514 |
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Description: Extracted from Panax ginseng C. A. Mey. dried roots;Store the product in sealed, cool and dry condition |
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Ginsenoside Rg2) 20(R)Ginsenoside Rg2 |
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| N2195-20 | ApexBio | 20 mg | EUR 224 |
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Description: Extracted from Panax ginseng C. A. Mey. dried roots;Store the product in sealed, cool and dry condition |
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Ginsenoside Rg3) 20(R)Ginsenoside Rg3 |
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| N2198-20 | ApexBio | 20 mg | EUR 514 |
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Description: Extracted from Panax ginseng C.A.Mey. roots;Store the product in sealed, cool and dry condition |
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 20UL FILTER TIPS FOR P-20 |
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| TF-20 | CORNING | 1000/pk | EUR 418 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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 AXYGEN® GEL TRAY 20 X 20 CM FOR USE WITH 20 CM GEL BOX, UV TRANSPARENT |
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| HGB20-20-GT | CORNING | 1/pk | EUR 97 |
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Description: Lab Equipment; Axygen Branded EQ |
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 DiagNano Silica-Coated Gold Nanoparticles, 20 nm |
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| CG-20 | Creative Diagnostics | 10mL | EUR 719 |
 AXYGEN® HORIZONTAL GEL BOX, 20 CM |
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| HGB-20 | CORNING | 1/pk | EUR 541 |
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Description: Lab Equipment; Axygen Branded EQ |
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) 3-D Life 10x CB Buffer (pH 7.2) |
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| B20-3 | Cellendes | 600 µL | EUR 44 |
 99447 DSSCT 20 X 125 W/ MARKING SPOT |
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| 99447-20 | CORNING | 250/pk | EUR 211 |
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Description: Disposable Screw Cap Culture Tubes; DSCCT's, Lab Stock |
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 DiagNano Gold Nanoparticle Passive Conjugation Kit, 20 nm |
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| GPK-20 | Creative Diagnostics | 1 kit | EUR 715 |
 DiagNano Silica-Coated PEGylated Gold Nanoparticles, 20 nm |
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| PG-20 | Creative Diagnostics | 10mL | EUR 719 |
 99449 DSSCT 20 X 125MM W/O MARKING SPOT |
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| 99449-20 | CORNING | 250/pk | EUR 228 |
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Description: Disposable Screw Cap Culture Tubes; DSCCT's, Lab Stock |
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 20 ul, autoclavable) Single channel mini-pipette (130 mm) 20 ul, autoclavable |
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| SCMP-20 | Alpha Diagnostics | 1 | EUR 92 |
 Recombinant Human Interleukin-20/IL-20 |
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| C147-10ug | Novoprotein | 10ug | EUR 202 |
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Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
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Recombinant Human Interleukin-20/IL-20 |
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| C147-1mg | Novoprotein | 1mg | EUR 2283 |
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Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
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Recombinant Human Interleukin-20/IL-20 |
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| C147-500ug | Novoprotein | 500ug | EUR 1613 |
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Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
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Recombinant Human Interleukin-20/IL-20 |
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| C147-50ug | Novoprotein | 50ug | EUR 496 |
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Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
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) Exosome binding buffer for ELISAs (20 ml) |
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| EXOEL-BB-20 | SBI | 20 ml | EUR 219 |
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) TumorExoRNA? Tumor-derived exosome immunocapture and RNA extraction kit (20 reactions) |
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| K1221-20 | Biovision | EUR 1219 | |
) ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions) |
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| M1030-20 | Biovision | EUR 1289 | |
) ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions) |
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| M1031-20 | Biovision | EUR 1289 | |
) TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions) |
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| M1032-20 | Biovision | EUR 1349 | |
) TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions) |
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| M1033-20 | Biovision | EUR 1349 | |
) MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 20 reactions) |
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| M1034-20 | Biovision | EUR 1246 | |
) MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 20 reactions) |
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| M1035-20 | Biovision | EUR 1246 | |
 IL-20 Interleukin-20 Mouse Recombinant Protein |
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| PROTQ9JKV9 | BosterBio | Regular: 10ug | EUR 317 |
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Description: Interleukin-20 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a total molecular mass of 17,584 Dalton. The IL-20 is purified by proprietary chromatographic techniques. |
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 IL-20 Interleukin-20 Human Recombinant Protein |
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| PROTQ9NYY1 | BosterBio | Regular: 10ug | EUR 317 |
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Description: Interleukin-20 Human Recombinant produced in E.Coli is a single, non-glycosylated homodimeric polypeptide chain containing 2 x 153 amino acids and having a total molecular mass of 35,212 Dalton. ;The IL-20 is purified by proprietary chromatographic techniques. |
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) MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 20 reactions) |
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| M1036-20 | Biovision | EUR 1289 | |
) MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 20 reactions) |
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| M1037-20 | Biovision | EUR 1289 | |
) ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 20 reactions) |
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| M1039-20 | Biovision | EUR 1289 | |
 Recombinant Murine FGF-acidic Protein |
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| PROTP61148-2 | BosterBio | 50ug | EUR 317 |
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Description: FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant murine FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues. |
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 Recombinant Rat FGF-acidic Protein |
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| PROTP61149-1 | BosterBio | 50ug | EUR 317 |
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Description: FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant rat FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues. |
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 Recombinant Human FGF-acidic Protein |
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| PROTP05230-2 | BosterBio | 50ug | EUR 317 |
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Description: FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGF-acidic is a 16.0 kDa protein consisting of 141 amino acid residues. |
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) ExoDNAPS? Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 20 reactions) |
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| K1230-20 | Biovision | EUR 718 | |
) ExoDNAUC? Circulating and Exosome-associated DNA Extraction Kit (Urine/Cell Media, 20 reactions) |
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| K1231-20 | Biovision | EUR 718 | |
 Recombinant Human Cytokeratin 20 |
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| 7-05461 | CHI Scientific | 5µg | Ask for price |
Recombinant Human Cytokeratin 20 |
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| 7-05462 | CHI Scientific | 20µg | Ask for price |
Recombinant Human Cytokeratin 20 |
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| 7-05463 | CHI Scientific | 1mg | Ask for price |
 Recombinant Human Interleukin-20 |
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| 7-01051 | CHI Scientific | 2µg | Ask for price |
Recombinant Human Interleukin-20 |
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| 7-01052 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Interleukin-20 |
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| 7-01053 | CHI Scientific | 1mg | Ask for price |
 Recombinant Mouse Interleukin-20 |
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| 7-01054 | CHI Scientific | 2µg | Ask for price |
Recombinant Mouse Interleukin-20 |
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| 7-01055 | CHI Scientific | 10µg | Ask for price |
Recombinant Mouse Interleukin-20 |
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| 7-01056 | CHI Scientific | 1mg | Ask for price |
 IL-20, human recombinant |
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| 4548-10 | Biovision | EUR 321 | |
IL-20, human recombinant |
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| 4548-1000 | Biovision | EUR 5917 | |
IL-20, human recombinant |
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| 4548-50 | Biovision | EUR 936 | |
 IL-20, murine recombinant |
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| 4549-10 | Biovision | EUR 283 | |
 IL-20, mouse recombinant |
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| 4549-1000 | Biovision | EUR 8532 | |
 FGF-20, human recombinant |
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| 4589-100 | Biovision | EUR 805 | |
FGF-20, human recombinant |
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| 4589-1000 | Biovision | EUR 3856 | |
Recombinant Human Interleukin-20 |
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| CC069-002 | GenDepot | 2ug | EUR 293 |
Recombinant Human Interleukin-20 |
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| CC069-010 | GenDepot | 10ug | EUR 408 |
Recombinant Human Interleukin-20 |
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| CC069-101 | GenDepot | 1mg | EUR 8960 |
Recombinant Mouse Interleukin-20 |
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| CC078-002 | GenDepot | 2ug | EUR 293 |
Recombinant Mouse Interleukin-20 |
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| CC078-010 | GenDepot | 10ug | EUR 408 |
Recombinant Mouse Interleukin-20 |
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| CC078-101 | GenDepot | 1mg | EUR 10310 |
) Recombinant Mucin 20 (MUC20) |
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| 4-RPA995Ra01 | Cloud-Clone |
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Description: Recombinant Rat Mucin 20 expressed in: E.coli |
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) Recombinant Keratin 20 (KRT20) |
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| 4-RPB240Hu01 | Cloud-Clone |
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Description: Recombinant Human Keratin 20 expressed in: E.coli |
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Recombinant Keratin 20 (KRT20) |
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| 4-RPB240Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Keratin 20 expressed in: E.coli |
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Recombinant Keratin 20 (KRT20) |
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| 4-RPB240Ra01 | Cloud-Clone |
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Description: Recombinant Rat Keratin 20 expressed in: E.coli |
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) Recombinant Interleukin 20 (IL20) |
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| 4-RPC058Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 20 expressed in: E.coli |
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) Recombinant Protocadherin 20 (PCDH20) |
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| 4-RPE100Hu01 | Cloud-Clone |
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Description: Recombinant Human Protocadherin 20 expressed in: E.coli |
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) Recombinant Cadherin 20 (CDH20) |
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| 4-RPG427Hu01 | Cloud-Clone |
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Description: Recombinant Human Cadherin 20 expressed in: E.coli |
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 20 bp random library with flanking sequence; DNA aptamer |
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| DAL-N-20 | Alpha Diagnostics | 100 ug | EUR 286 |
 DiagNano Silica-Coated Gold Nanoparticles, 20 nm, 100 OD |
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| CG-20-100 | Creative Diagnostics | 0.25mL | EUR 772 |
 DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 20 nm |
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| GCK-M-20 | Creative Diagnostics | 1 kit | EUR 757 |
 DiagNano Fluorophore Labeled Gold Nanoparticles, 20 nm, Cellular Uptake |
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| GFL-20-CU | Creative Diagnostics | 1mL | EUR 1365 |
) Custom Anti-Peptide antibodies (peptide synthesis up to 20-aa, conjugation, 2 rabbits, ELISA) |
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| ABPEP-20 | Alpha Diagnostics | 1 | EUR 1626 |
) TotalExoRNA? Overall exosome immunocapture and RNA extraction kit (Biological Fluids/Cell Media, 20 reactions) |
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| K1220-20 | Biovision | EUR 1219 | |
 DiagNano Silica-Coated PEGylated Gold Nanoparticles, 20 nm, 100 OD |
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| PG-20-100 | Creative Diagnostics | 0.25mL | EUR 918 |
20 Coated Gold Particles) Oligo (dT)20 Coated Gold Particles |
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| OdT(20)AuP-002-1 | Spherotech | 1 mL | EUR 385 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Oligo (dT)20 Coated Gold Particles |
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| OdT(20)AuP-002-5 | Spherotech | 5 mL | EUR 1045 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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 Recombinant Human Fibroblast Growth Factor-acidic |
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| 7-00319 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Fibroblast Growth Factor-acidic |
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| 7-00320 | CHI Scientific | 50µg | Ask for price |
Recombinant Human Fibroblast Growth Factor-acidic |
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| 7-00321 | CHI Scientific | 1mg | Ask for price |
 Recombinant Mouse Fibroblast Growth Factor-acidic |
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| 7-00325 | CHI Scientific | 10µg | Ask for price |
Recombinant Mouse Fibroblast Growth Factor-acidic |
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| 7-00326 | CHI Scientific | 50µg | Ask for price |
Recombinant Mouse Fibroblast Growth Factor-acidic |
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| 7-00327 | CHI Scientific | 1mg | Ask for price |
 FGF-1/FGF-acidic, human recombinant |
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| 4034-10 | Biovision | EUR 137 | |
FGF-1/FGF-acidic, human recombinant |
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| 4034-1000 | Biovision | EUR 1675 | |
FGF-1/FGF-acidic, human recombinant |
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| 4034-50 | Biovision | EUR 256 | |
 FGF-1/FGF-acidic, murine recombinant |
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| 4035-10 | Biovision | EUR 137 | |
FGF-1/FGF-acidic, murine recombinant |
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| 4035-1000 | Biovision | EUR 1675 | |
FGF-1/FGF-acidic, murine recombinant |
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| 4035-50 | Biovision | EUR 256 | |
 Recombinant human Acidic repeat-containing protein |
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| P2170 | FN Test | 100ug | Ask for price |
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Description: Recombinant protein for human Acidic repeat-containing protein |
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) Recombinant Glial Fibrillary Acidic Protein (GFAP) |
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| 4-RPA068Hu01 | Cloud-Clone |
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Description: Recombinant Human Glial Fibrillary Acidic Protein expressed in: E.coli |
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Recombinant Glial Fibrillary Acidic Protein (GFAP) |
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| 4-RPA068Hu02 | Cloud-Clone |
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Description: Recombinant Human Glial Fibrillary Acidic Protein expressed in: E.coli |
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Recombinant Glial Fibrillary Acidic Protein (GFAP) |
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| 4-RPA068Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Glial Fibrillary Acidic Protein expressed in: E.coli |
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Recombinant Glial Fibrillary Acidic Protein (GFAP) |
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| 4-RPA068Mu02 | Cloud-Clone |
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Description: Recombinant Mouse Glial Fibrillary Acidic Protein expressed in: E.coli |
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Recombinant Glial Fibrillary Acidic Protein (GFAP) |
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| 4-RPA068Mu03 | Cloud-Clone |
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Description: Recombinant Mouse Glial Fibrillary Acidic Protein expressed in: E.coli |
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Recombinant Glial Fibrillary Acidic Protein (GFAP) |
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| 4-RPA068Mu04 | Cloud-Clone |
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Description: Recombinant Mouse Glial Fibrillary Acidic Protein expressed in: E.coli |
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Recombinant Glial Fibrillary Acidic Protein (GFAP) |
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| 4-RPA068Ra01 | Cloud-Clone |
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Description: Recombinant Rat Glial Fibrillary Acidic Protein expressed in: E.coli |
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Recombinant Glial Fibrillary Acidic Protein (GFAP) |
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| 4-RPA068Ra02 | Cloud-Clone |
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Description: Recombinant Rat Glial Fibrillary Acidic Protein expressed in: E.coli |
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 Recombinant human FGF acidic/FGF1 Protein |
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| RP01242 | Abclonal | 10 μg | EUR 149 |
 StayBrite? Highly Stable Luciferase |
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| 7901-150 | Biovision | EUR 387 | |
Precautionary resistance administration technique needs to be carried out. The phenomenon described within the examine may have broader organic significance.