Product Details
Kind: Mouse IgG1
Specificity
The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), asialoglycoprotein expressed in early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes, and cells of the K562 erythroid cell lines. HEL. The antibody does not react with glycophorin B.
Request
FC (quality control tested)
Details of the application
Flow Cytometry – The reagent is designed for the analysis of human blood cells using 4 μl of reagent / 100 μl of whole blood or 106 cells in suspension. The content of one vial (0.4 ml) is sufficient for 100 tests.
Reactivity: Rat, Human
Immunogen
Splenic Hairy Cell Leukemia Membrane Preparation
Preparation
The purified antibody is conjugated to fluorescein isothiocyanate (FITC) under optimal conditions. The conjugate is purified by size exclusion chromatography.
Formulation
Phosphate Buffered Stabilizing Saline (PBS) containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8 ° C. Do not freeze. Avoid prolonged exposure to light. Do not use after the expiration date printed on the vial label.
The product is designed for research use only. Diagnostic or therapeutic applications are strictly prohibited. The products must not be used for resale or transfer to third parties, either as a standalone product or as a manufacturing component of another product without the written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be responsible for patent infringement or any other infringement of intellectual property rights that may occur with the use of the products.
Other names
- Glycophorin A
- GYPA
- GPA
- PAS-2
- Sialoglycoprotein alpha
- MN sialoglycoprotein
- GPSAT
- GPErik
- MSN blood group
Description of the antigen
CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed in erythrocytes and their precursors. Similar to glycophorin B (GPB), these molecules provide cells with a large mucin-like surface area, which minimizes aggregation between circulating erythrocytes. GPA is the carrier of the M and N blood group specificities, while GPB represents the S, s, and U specificities. CD235a is a receptor for Hsa, a Streptococcus adhesin.
Entrez gene ID: 2993
UniProt ID: P02724
