Unlike the lectins, Ulex europaeus and Lotus tetragonolobus which prefer bound fucose residues (α -1.2), the lectin Aleuria aurantia preferentially binds to bound fucose (α -1.6) to N-acetylglucosamine or to bound fucose (α -1,3) to structures related to N-acetylactosamine. ALA also binds reversibly to nucleic acid-bound fucose.
Biotinylated Aleuria Aurantia lectin has an appropriate number of biotins attached to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Unit size: 1 mg
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Transfer Applications, Elispot, ELISA, Glycobiology
Recommended storage: 2-8 ° C; Store frozen for long-term storage
Inhibition and/or elution of sugar: 100-400 mM L-fucose
Solution: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Concentration: 2 mg active conjugate / ml
Specificity of Sugae: fucose, arabinose